50 Preps, 200 Preps

Le kit isebenzisa i-spin ikholomu kanye nefomulaithuthukiswe inkampani yethu, engakwazi ukukhipha i-RNA ehlanzekile nesezingeni eliphezulu ezicutshini zezilwane ezihlukahlukene ngokusebenza kahle okuphezulu.Ihlinzeka ngekholomu ye-DNA-Cleaning ephumelelayo, engahlukanisa kalula futhi ikhangise i-DNA ye-genomic ku-supernatant kanye ne-tissue lysate, elula. kanye nokonga isikhathi;Ikholomu ye-RNA kuphela ingahlanganisa ngempumelelo i-RNA futhi ingacutshungulwa kanyekanye ngefomula eyingqayizivele Amasampuli amaningi.

Lonke uhlelo luyi-RNase-Free, ukuze i-RNA ekhishiwe ingonakalisiwe;I-Buffer RW1, i-Buffer RW2 isistimu yokugeza ibhafa, ukuze i-RNA etholiwe ingabi nawo amaprotheni, i-DNA, i-ion, kanye nokungcoliswa kwenhlanganisela yezinto eziphilayo.

Izingxenye zekhithi

Ulwazi lomkhiqizo

Izici&izinzuzo

■ Asikho isidingo sokukhathazeka ngokuwohloka kwe-RNA;lonke uhlelo luyi-RNase-Free
■ Khipha ngempumelelo i-DNA usebenzisa i-DNA-Cleaning Column
■ Khipha i-DNA ngaphandle kokwengeza i-DNase
■ Kulula-yonke imisebenzi iqedwa ekamelweni lokushisa
■ Ukusebenza okusheshayo kungaqedwa ngemizuzu engama-30
■ I-Safe-akukho reagent ephilayo edingekayo
■ Ukuhlanzeka okuphezulu -OD260/280≈1.8-2.1

Isicelo sekhithi

Ilungele ukukhishwa nokuhlanzwa kwe-RNA ephelele ezinhlobonhlobo zezicubu zezilwane ezintsha noma eziqandisiwe noma amaseli akhulisiwe.

Imingcele yomkhiqizo

■ Izinhlelo zokusebenza eziwela phansi: ukuhlanganiswa kwe-cDNA ye-first-strand, i-RT-PCR, i-molecular cloning, i-Northern Blot, njll.
■ Amasampula: izicubu zezilwane, amaseli akhulisiwe
■ Umthamo: Izicubu 10-20mg, Amaseli(2-5)×10⁶
■ Umthamo omkhulu wokubopha we-DNA wekholomu yokuhlanza: 80 μg
■ Ivolumu ye-Elution: 50-200 μl

Ukugeleza komsebenzi

I-Animal Total RNA Isolation Kit iphathwe ama-20mg
Amasampula egundane amasha, thatha i-RNA ephelele engu-5% 1% ye-agar

I-Glycogel electrophoresis
1: Ubende 2: Izinso
3: Isibindi 4: Inhliziyo

Isitoreji nempilo yeshelufu

Ikhithi ingagcinwa izinyanga ezingama-24 ekamelweni lokushisa (15-25 ℃) noma 2–8 ℃ isikhathi eside.I-Buffer RL1 ingagcinwa ku-4 ℃ inyanga engu-1 ngemva kokwengeza i-β- mercaptoethanol (uma uthanda).

 

Izihloko ezicashuniwe

1.IF:18.808:Zheng, Q., Qin, F., Luo, R., et al.I-mRNA-Layishwa I-Lipid-Like Nanoparticles Yokuhlelwa Kwesisekelo Sesibindi Ngokuthuthukiswa Komklamo Oyinhlanganisela Emaphakathi.Adv.Umsebenzi.UMater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.IF:18.187:He X, Hong W, Yang J, et al.I-apoptosis ezenzakalelayo yamaseli ekulungiseleleni i-stem cell therapy isebenzisa imiphumela yokuzivikela ngokukhululwa kwe-phosphatidylserine.I-Signal Transduct Target Ther.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.IF:17.97 ai Z, Liu H, Liao J, et al.Ukuguqulwa kwe-N7-Methylguanosine tRNA kuthuthukisa ukuhumusha kwe-oncogenic mRNA futhi kukhuthaze ukuqhubeka kwe-intrahepatic cholangiocarcinoma.Mol Cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.IF:9.225:Cao X, Shu Y, Chen Y, et al.Ukuguqulwa kwe-M6A kwe-Mettl14-Mediated Kusiza Ukuvuselelwa Kwesibindi Ngokugcina I-Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

I-RNA isoaltion kits ye eminye imithombo yesampulaayatholakala:

Iseli, isitshalo, igciwane, igazi, njll.

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Okwedlule: I-Cell Direct RT QPCR Kit—SYBR GREEN I

Olandelayo: Umkhiqizi ukhiqiza ifilimu ye-anti-sun reflection

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