ISeli eNgqo ngqo yeRT QPCR Kit—SYBR GREEN I

Intshayelelo

◮Ilula kwaye iyasebenza: ngeTekhnoloji ye-Cell Direct RT, iisampulu ze-RNA zinokufumaneka kwimizuzu eyi-7 nje.

Imfuno yesampulu incinci, iphantsi njengeeseli ezili-10 zinokuvavanywa.

◮Ukuphumelela okuphezulu: inokubona ngokukhawuleza i-RNA kwiiseli ezikhuliswe kwii-384, 96, 24, 12, 6-well plates.

I-DNA Eraser inokususa ngokukhawuleza i-genomes ekhutshiweyo, inciphise kakhulu impembelelo kwiziphumo zovavanyo ezilandelayo.

Inkqubo ye-RT elungiselelweyo kunye ne-qPCR yenza amanyathelo amabini e-RT-PCR aguqulelwe umva asebenze ngakumbi kwaye i-PCR icace ngakumbi, kwaye ixhathisa ngakumbi kwi-RT-qPCR reaction inhibitors.

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo enokuthi ikhulule ngokukhawuleza i-RNA kwiisampulu zeseli ezikhuliswe kwiimpendulo ze-RT-qPCR, ngaloo ndlela iphelisa inkqubo yokucoca i-RNA ethatha ixesha kunye nenzima.Ithemplethi ye-RNA inokufumaneka kwimizuzu nje esi-7.Umxube we-RT oyi-5xDirect kunye ne-2×Direct qPCR Mix-SYBR ii-reagents ezibonelelwe yikhithi zinokufumana ngokukhawuleza nangempumelelo iziphumo zePCR zexesha lokwenyani.

I-5 × Direct RT Mix kunye ne-2 × Direct qPCR Mix-SYBR inokunyamezela inhibitor eqinile, kunye ne-lysate yeesampuli zingasetyenziswa njenge template ye-RT-qPCR ngokuthe ngqo.Le khithi iqulethe i-RNA ephezulu ehambelana ne-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.

Iinkcukacha

200×20μl Rxns, 1000×20μl Rxns

Amacandelo ekhithi

Icandelo I

Isithinteli CL

Foregene Protease Plus II

Isithinteli ST

Icandelo II

DNA Eraser

5 × Ngqo RT Mix

2 × Ngqo qPCR Mix-SYBR

I-50 × i-ROX Reference Dye

RNase-Free ddH2O

Imiyalelo

Iimpawu&izinto eziluncedo

■ Ilula kwaye isebenzayo : ngeteknoloji ye-Cell Direct RT, iisampuli ze-RNA zinokufumaneka kwimizuzu nje eyi-7.

∎ Imfuno yesampulu incinci, ingaphantsi njengeeseli ezili-10 zinokuvavanywa.

■ Ukugqithisa okuphezulu: inokubona ngokukhawuleza i-RNA kwiiseli ezikhuliswe kwii-384, 96, 24, 12, 6-well plates.

■ I-DNA Eraser inokususa ngokukhawuleza iigenomes ezikhutshiweyo, inciphise kakhulu impembelelo kwiziphumo zovavanyo ezilandelayo.

■ Inkqubo elungiselelwe i-RT kunye ne-qPCR yenza amanyathelo amabini e-RT-PCR akhutshelwe umva asebenze ngakumbi kwaye i-PCR icace ngakumbi, kwaye imelane ngakumbi ne-RT-qPCR reaction inhibitors.

Isicelo seKit

Ububanzi besicelo: iiseli ezikhuliswe.

- I-RNA ekhutshwe ngesampuli ye-lysis: isebenza kuphela kwi-template ye-RT-qPCR yale khithi.

- Ikiti ingasetyenziselwa ezi njongo zilandelayo: uhlalutyo lwe-gene expression, ukuqinisekiswa kwe-siRNA-mediated gene silence effect, ukuhlolwa kweziyobisi, njl.

Umzobo

I-Cell-Direct-RT-qPCR-diagram

 

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-4℃;Icandelo II kufuneka ligcinwe kwi -20 ℃.

I-Foregene Protease Plus II kufuneka igcinwe ku-4℃, ungakhenkcezisi ku -20℃.

I-Reagent 2 × Direct qPCR Mix-SYBR kufuneka igcinwe kwi -20 ℃ ebumnyameni;ukuba isetyenziswe rhoqo, inokugcinwa kwi-4 ℃ yokugcina ixesha elifutshane (sebenzisa phakathi kweentsuku ezili-10).


  • Ngaphambili:
  • Okulandelayo:

  • Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    Injineli yobuchwephesha obuchwepheshile ezinikele ukukukhokela

    Ngokweemfuno zakho zokwenyani, khetha eyona ndlela ifanelekileyo yoyilo kunye neenkqubo zokucwangcisa