50 Preps, 200 Preps

Sekhahla sena se sebetsaspin kholomo le foromoe ntlafalitsoeng ke k'hamphani ea rona, e ka ntšang RNA ea boleng bo holimo le ea boleng bo holimo ho tsoa liseleng tse fapaneng tsa liphoofolo tse nang le ts'ebetso e phahameng. le ho boloka nako;Kholomo ea RNA-feela e ka tlama RNA hantle 'me e ka sebetsoa ka nako e le 'ngoe ka foromo e ikhethang Mehlala e mengata.

Tsamaiso eohle ke RNase-Free, e le hore RNA e nkiloeng e se ke ea senyeha;Buffer RW1, Buffer RW2 buffer system ea ho hlatsoa, ​​e le hore RNA e fumanoeng e se na protheine, DNA, ion, le tšilafalo ea motsoako oa organic.

Likarolo tsa Kit

Lintlha tsa sehlahisoa

Likarolo le melemo

■ Ha ho hlokahale hore u tšoenyehe ka ho senyeha ha RNA;tsamaiso eohle ke RNase-Free
■ Tlosa ka katleho DNA-sebelisa DNA-Cleaning Column
■ Tlosa DNA ntle le ho eketsa DNase
■ Ts'ebetso e bonolo-tsohle e phethoa ka mocheso oa kamore
■ Ts'ebetso e potlakileng e ka phethoa ka mor'a metsotso e 30
■ Sireletsehile-ha ho na reagent ea tlhaho e hlokahalang
■ Bohloeki bo phahameng -OD260/280≈1.8-2.1

Sesebelisoa sa Kit

E loketse ho ntšoa le ho hloekisoa ha RNA e felletseng ho tsoa mefuteng e fapaneng ea lithane tsa liphoofolo tse foreshe kapa tse hatselitsoeng kapa lisele tse holileng.

Lihlahisoa tsa lihlahisoa

■ Lisebelisoa tse tlase: motsoako oa pele oa cDNA, RT-PCR, cloning ea molek'hule, Northern Blot, joalo-joalo.
■ Mehlala: lisele tsa liphoofolo, lisele tse hōlileng tsebong
■ Tekanyetso: Li-tissues 10-20mg, Lisele (2-5)×10⁶
■ Boholo bo tlamang DNA ea kholomo ea tlhoekiso: 80 μg
■ Molumo oa elution: 50-200 μl

Phallo ea mosebetsi

Animal Total RNA Isolation Kit tšoaroa 20mg
Fresh mouse disampole, nka 5% hloekisitsoeng kakaretso RNA 1% agar

Glycogel electrophoresis
1: Lesapo la 2: Liphio
3: Sebete 4: Pelo

Polokelo le shelf bophelo

Thepa e ka bolokoa likhoeli tse 24 mocheso oa kamore (15-25 ℃) kapa 2-8 ℃ nako e telele.Buffer RL1 e ka bolokoa ho 4 ℃ bakeng sa khoeli ea 1 ka mor'a ho eketsa β- mercaptoethanol (ho ikhethela).

 

Lingoloa tse qotsitsoeng

1.HAEBA:18.808:Zheng, Q., Qin, F., Luo, R., et al.mRNA-Loaded Lipid-Like Nanoparticles bakeng sa Liver Base Editing ka Ntlafatso ea Moqapi o Bohareng oa Composite.Adv.Mosebetsi.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.HAEBA:18.187:He X, Hong W, Yang J, et al.Apoptosis e iketsang ea lisele tsa kalafo ea stem cell e fana ka litlamorao tsa immunomodulatory ka ho lokolloa ha phosphatidylserine.Signal Transduct Target Ther.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.HAEBA:17.97 ai Z, Liu H, Liao J, et al.Phetoho ea N7-Methylguanosine tRNA e ntlafatsa phetolelo ea oncogenic mRNA hape e khothalletsa tsoelo-pele ea intrahepatic cholangiocarcinoma.Mol Cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.HAEBA:9.225:Cao X, Shu Y, Chen Y, et al.Mettl14-Mediated m6A Phetoho e Thusa ho Tsosolosa Sebete ka ho Boloka Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

RNA isoaltion kits bakeng sa mehloli e meng ea mohlalalia fumaneha:

Sele, semela, kokoana-hloko, mali, joalo-joalo.

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E fetileng: Cell Direct RT QPCR Kit—SYBR GREEN I

E 'ngoe: Moetsi o hlahisa filimi e khahlanong le letsatsi

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