50 Diyaarin, 200 Diyaarin

Qalabkani waxa uu isticmaalaalafdhabarta iyo caanahaoo ay soo saartay shirkadeena, taas oo ka soo saari karta wadarta guud ee RNA-nadiifinta sare iyo tayada sare leh ee unugyada xoolaha kala duwan oo leh waxtar sare leh.Waxay bixisaa DNA-Nadiifinta Tiirka wax ku ool ah, kaas oo si fudud u kala saari kara oo u xayeysiin kara DNA-ga genomic-ka sare iyo unugyada lysate, fudud. iyo waqti-badbaadinta;Tiirka RNA-keliya ayaa si hufan u xidhi kara RNA waxaana lagu farsamayn karaa qaacido gaar ah Tiro badan oo muunado ah.

Nidaamka oo dhan waa RNase-free, si RNA-ga la soo saaray aanu hoos u dhicin;Buffer RW1, Buffer RW2 nidaamka dhaqidda bakhaar, si RNA-ga la helay uu xor uga ahaado borotiinka, DNA, ion, iyo wasakheynta isku dhafka dabiiciga ah.

Qaybaha kitka

Macluumaadka alaabta

Tilmaamaha & faa'iidooyinka

■ Looma baahna in laga walwalo xaalufka RNA;nidaamka oo dhan waa RNase-free
■ Si wax ku ool ah uga saar DNA-da adoo isticmaalaya Tiirka Nadiifinta DNA
■ Ka saar DNA adigoon ku darin DNA-da
■ Hawlgallada fudud-dhammaan waxaa lagu dhammeeyaa heerkulka qolka
■ Hawlgalka degdegga ah waxa lagu dhamayn karaa 30 daqiiqo
■ Badbaado-ma baahna reageent organic ah
■ Nadaafadda sare -OD260/280≈1.8-2.1

Codsiga qalabka

Waxay ku habboon tahay soo saarista iyo nadiifinta wadarta RNA ee noocyo kala duwan oo unugyo xayawaan ah oo cusub ama barafaysan ama unugyo dhaqan leh.

Xuduudaha alaabta

■ Codsiyada hoose: isku dhafka cDNA ee ugu horreeya, RT-PCR, cloning molecular, Northern Blot, iwm.
Tusaalooyinka: unugyada xoolaha, unugyo dhaqan
■ Qiyaasta: Unugyada 10-20mg, Unugyada (2-5)×10⁶
■ Awoodda ugu badan ee DNA-da ee tiirka sifaynta: 80 μg
■ Mugga iftiinka: 50-200 μl

Socodka shaqada

Wadarta Xayawaanka Go'doominta RNA ee lagu daweeyay 20mg
Muuska cusub, qaado 5% wadarta guud ee RNA la safeeyey 1% agar

Glycogel electrophoresis
1: beeryarada 2: kelyaha
3: Beerka 4: Wadnaha

Kaydinta iyo nolosha shelf

Qalabka waxaa lagu kaydin karaa 24 bilood heerkulka qolka (15-25 ℃) ama 2-8 ℃ wakhti dheer.Buffer RL1 waxa lagu kaydin karaa 4 ℃ 1 bil kadib marka lagu daro β-mercaptoethanol (ikhtiyaar).

 

Maqaallo la soo xigtay

1.HADDII:18.808:Zheng, Q., Qin, F., Luo, R., iyo al.mRNA-Loaded Lipid-Lipid Nanoparticles oo loogu talagalay Tafatirka Saldhigga Beerka iyadoo loo marayo Hagaajinta Naqshadaynta Isku-dhafka Dhexe.Adv.ShaqaaleMater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.HADDII:18.187:He X, Hong W, Yang J, iyo al.apoptosis kediska ah ee unugyada ku jira diyaarinta unugyada asliga ah ee daweynta waxay sameeyaan saameyn immunomodulatory ah iyada oo la sii daayo fosfatidylserine.Bartilmaameedka Is-beddelka Signal Ther.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.HADDII:17.97 ai Z, Liu H, Liao J, iyo al.N7-Methylguanosine tRNA wax ka beddelka waxay wanaajisaa tarjumaada mRNA oncogenic waxayna kor u qaadaa horumarka cholangiocarcinoma intrahepatic.Mol Cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.HADDII:9.225:Cao X, Shu Y, Chen Y, iyo al.Mettl14-Mediated m6A Beddelka ayaa Fududeeya Dib u soo Kabashada Beerka iyadoo la ilaalinayo Endoplasmic Reticulum Homeostasis.Unugyada Mol Gastroenterol Hepatol.2021;12 (2): 633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

Xirmooyinka go'doominta RNA ee ilo muunado kalewaa la heli karaa:

Unug, dhir, fayras, dhiig, iwm.

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Hore: Qalabka Tooska ah ee Unugga RT QPCR-SYBR GREEN I

Xiga: Soo saaraha ayaa soo saara filim ka soo horjeeda qoraxda

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