Iyi kit inoshandisa yakasarudzika lysis buffer sisitimu inogona kukurumidza kuburitsa RNA kubva kune yakasimwa maseru masampula eRT-qPCR maitiro, nekudaro ichibvisa iyo inopedza nguva uye yakaoma RNA yekuchenesa maitiro.Iyo RNA template inogona kuwanikwa mumaminetsi manomwe chete.Iyo 5 × Direct RT Musanganiswa uye 2 × Direct qPCR Musanganiswa-SYBR reagents anopihwa nekiti anogona nekukurumidza uye zvinobudirira kuwana chaiyo-nguva yehuwandu hwePCR mhinduro.

5 × Yakananga RT Mix uye 2 × Yakananga qPCR Mix-SYBR ine inhibitor kushivirira kwakasimba, uye lysate yemasampuli inogona kushandiswa se template yeRT-qPCR zvakananga.Iyi kit ine yakasarudzika RNA yakakwirira-yakabatana Foregene reverse transcriptase, uye Inopisa D-Taq DNA polymerase, dNTPs, MgCl.₂, reaction buffer, PCR optimizer uye stabilizer.

200×20μl Rxns, 1000×20μl Rxns

■ Zviri nyore uye zvinoshanda : neTekinoroji yeCell Direct RT, masampuli eRNA anogona kuwanikwa mumaminetsi manomwe chete.

∎ Sample kudiwa idiki, yakaderera semaseru gumi anogona kuongororwa.

■ High throughput: inogona kukurumidza kuona RNA mumaseru akagadzirwa mu384, 96, 24, 12, 6-tsime mahwendefa.

∎ DNA Eraser inogona kukurumidza kubvisa majenomes akaburitswa, kuderedza zvakanyanya kukanganiswa pamhedzisiro yekuedza.

■ Optimized RT uye qPCR sisitimu inoita kuti nhanho mbiri RT-PCR reverse transcription inyatsoita uye PCR yakanyatsojeka, uye inoshingirira kuRT-qPCR reaction inhibitors.

Kuwanda kwekushandiswa: masero akagadzirwa.

- RNA yakaburitswa nesample lysis: inoshanda chete kune RT-qPCR template yeiyi kit.

- Iyo kit inogona kushandiswa kune zvinotevera zvinangwa: gene kutaura kuongororwa, kuoneswa kweSiRNA-mediated gene kunyaradza maitiro, kuongorora zvinodhaka, nezvimwe.