50 Preps, 200 Preps

Iyi kit inoshandisa iyospin column uye formulayakagadziridzwa nekambani yedu, iyo inogona kubvisa yakakwirira-kuchena uye yakakwirira-yemhando yakazara RNA kubva kune akasiyana matishu emhuka ane hunyanzvi hwepamusoro.Inopa inoshanda DNA-Kuchenesa Column, iyo inogona kuparadzanisa nyore uye adsorb genomic DNA kubva kune supernatant uye tissue lysate, nyore. uye kuchengetedza nguva;RNA-chete Column inokwanisa kusunga RNA zvakanaka uye inogona kugadziriswa panguva imwe chete neyakasarudzika fomula Mazhinji emasamples.

Iyo yese sisitimu ndeye RNase-Yemahara, kuitira kuti iyo yakabviswa RNA irege kuderedzwa;Buffer RW1, Buffer RW2 buffer washing system, kuitira kuti iyo RNA inowanikwa haina mapuroteni, DNA, ion, uye organic compound kusvibiswa.

Kit zvikamu

Ruzivo rwechigadzirwa

Zvimiro&zvakanakira

■ Hapana chikonzero chekunetseka pamusoro pekuparara kweRNA;iyo yese system ndeye RNase-Yemahara
■ Bvisa DNA-uchishandisa DNA-Cleaning Column
■ Bvisa DNA pasina kuwedzera DNase
■ Kushanda kuri nyore-zvose zvinopedzwa patembiricha yepakamuri
■ Kukurumidza-kushanda kunogona kupedzwa mumaminitsi makumi matatu
■ Yakachengeteka-hapana organic reagent inodiwa
■ Kuchena kwepamusoro -OD260/280≈1.8-2.1

Kit application

Inokodzera kudhirowa uye kucheneswa kweiyo RNA yakazara kubva kune akasiyana siyana matsva kana akaomeswa mhuka mhuka kana maseru ane tsika.

Product parameters

■ Zvishandiso zvepasi: yekutanga-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, nezvimwe.
∎ Mienzaniso: matishu emhuka, masero ane tsika
■ Dosage: Matishu 10-20mg, Masero(2-5)×10⁶
■ Maximum DNA inosunga simba rekuchenesa column: 80 μg
■ Elution volume: 50-200 μl

Kuyerera kwebasa

Mhuka Yese RNA Isolation Kit yakarapwa 20mg
Fresh mbeva samples, tora 5% yakacheneswa yakazara RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: itsvo
3: Chiropa 4: Mwoyo

Kuchengetedza uye hupenyu hwesherufu

Iyo kit inogona kuchengetwa kwemwedzi makumi maviri nemana pamhepo tembiricha (15-25 ℃) kana 2-8 ℃ kwenguva yakareba.Buffer RL1 inogona kuchengetwa pa4 ℃ kwemwedzi 1 mushure mekuwedzera β- mercaptoethanol (kusarudza).

 

Nyaya dzakataurwa

1.IF:18.808:Zheng, Q., Qin, F., Luo, R., et al.mRNA-Yakatakura Lipid-Senge Nanoparticles yeLiver Base Editing kuburikidza neKugadziridza kweCentral Composite Design.Adv.Funct.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.IF:18.187:Iye X, Hong W, Yang J, nevamwe.Spontaneous apoptosis yemasero mune yekurapa stem cell kugadzirira inoshandisa immunomodulatory mhedzisiro kuburikidza nekuburitswa kwephosphatidylserine.Signal Transduct Target Ther.2021 Jul 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.IF:17.97 ai Z, Liu H, Liao J, nevamwe.N7-Methylguanosine tRNA kugadziriswa kunowedzera oncogenic mRNA shanduro uye inosimudzira intrahepatic cholangiocarcinoma kufambira mberi.Mol Cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.KANA:9.225:Cao X, Shu Y, Chen Y, et al.Mettl14-Mediated m6A Kugadziriswa Kunofambisa Chiropa Kuberekwazve neKuchengetedza Endoplasmic Reticulum Homeostasis.Sero Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

RNA isoaltion kits ye mamwe sampuli masosizviripo:

Sero, chirimwa, utachiona, ropa, zvichingodaro.

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Zvakapfuura: Sero Yakananga RT QPCR Kit-SYBR GREEN I

Zvinotevera: Mugadziri anogadzira anti-sun reflection film

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