50 Sauniuniga, 200 Sauniuniga

O lenei pusa e faʻaaogaina levili koluma ma fua fa'atatauatiina ae e la tatou kamupani, lea e mafai ona maua mai le maualuga-mama ma le maualuga o le RNA atoa mai le tele o aano o manu ma le lelei maualuga. E maua ai se DNA-Cleaning Column, lea e mafai ona faigofie ona vavae ese ma faʻapipiʻi DNA genomic mai le supernatant ma le lysate tissue, faigofie. ma le faasaoina o le taimi;RNA-na'o le Column e mafai ona fusifusia lelei le RNA ma e mafai ona fa'agasolo fa'atasi ma se fua fa'apitoa Tele fa'ata'ita'iga.

O le faiga atoa e RNase-Free, ina ia le faʻaleagaina le RNA na maua mai;Buffer RW1, Buffer RW2 faʻapipiʻi fufulu faiga, ina ia maua le RNA e saʻoloto mai le polotini, DNA, ion, ma le faʻaleagaina o mea faʻalaʻau.

Vaega o pusa

Fa'amatalaga o oloa

Fa'ailoga & fa'amanuiaga

■ Leai se mea e popole ai e uiga i le faaleagaina o le RNA;ole faiga atoa ole RNase-Free
■ Aveese lelei le DNA-faʻaaogaina le DNA-Cleaning Column
■ Aveese le DNA e aunoa ma le faaopoopoina o le DNase
■ Faigofie-o faagaioiga uma e faamaeaina i le vevela o le potu
■ Saosaoa -e mafai ona faamaeaina i le 30 minute
■ Saogalemu-e le mana'omia se reagent organic
■ maualuga mama -OD260/280≈1.8-2.1

Fa'aoga pusa

E talafeagai mo le aveeseina ma le faʻamamaina o le RNA atoa mai le tele o mea fou pe faʻaisa manu aano poʻo sela faʻaleaganuʻu.

Fa'atauga o oloa

■ Talosaga i lalo: fa'asologa muamua o le cDNA, RT-PCR, mole mole, Blot i Matu, ma isi.
■ Fa'ata'ita'iga: aano o manu, sela fa'atupuina
■ Fuafuaga: Tissue 10-20mg, Cells(2-5)×10⁶
■ Ole maualuga ole gafatia ole fusifusia ole DNA ole koluma fa'amamaina: 80 μg
■ Elution voluma: 50-200 μl

Fa'agasologa o galuega

Manu Aofa'i RNA Isolation Kit togafitia 20mg
Fa'ata'ita'iga o isumu fou, ave 5% fa'amama atoa RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Fatugao
3: Ate 4: Loto

Teuina ma le ola fa'asa

E mafai ona teu le pusa mo le 24 masina i le vevela o le potu (15-25 ℃) poʻo le 2-8 ℃ mo se taimi umi.E mafai ona teuina le Buffer RL1 i le 4 ℃ mo le 1 masina pe a uma ona fa'aopoopo le β-mercaptoethanol (filifiliga).

 

Tala fa'atatau

1.IF:18.808:Zheng, Q., Qin, F., Luo, R., et al.mRNA-Loaded Lipid-Like Nanoparticles mo Fa'ata'ita'iga Fa'avae A'ai E ala i le Fa'ata'ita'iina o le Fa'asologa Fa'atasiga Tutotonu.Adv.Galuega.Mata.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.IF:18.187:He X, Hong W, Yang J, ma isi.O le apoptosis faʻafuaseʻi o sela i le sauniuniga o le cell stem e faʻaalia ai aʻafiaga immunomodulatory e ala i le tuʻuina atu o le phosphatidylserine.Fa'ailo Fa'ailo Fa'atatau i ai.2021 Iulai 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.IF:17.97 ai Z, Liu H, Liao J, et al.N7-Methylguanosine tRNA suiga e faʻaleleia ai le faʻaliliuga mRNA oncogenic ma faʻateleina le alualu i luma o le cholangiocarcinoma intrahepatic.Cell mol.2021 Iul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.AFAI:9.225: Cao X, Shu Y, Chen Y, et al.Mettl14-Mediated m6A Suiga e Faʻafaigofie le Faʻafouina o le Atoʻo e ala i le Tausia o le Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

RNA va'aiga pusa mo isi punaoa faʻataʻitaʻigao lo'o avanoa:

Cell, laau, viral, toto, ma isi.

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Muamua: Cell Direct RT QPCR Kit—SYBR GREEN I

Sosoo ai: O lo'o gaosia e le kamupani gaosi mea e tete'e i le la

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