Iki gikoresho gikoresha sisitemu yihariye ya lysis ishobora kurekura vuba RNA ingero zumuco zumuco kubitekerezo bya RT-qPCR, bityo bikuraho inzira yo kweza RNA itwara igihe kandi ikora.Inyandikorugero ya RNA irashobora kuboneka muminota 7 gusa.5 × Direct RT ivanze na 2 × Direct qPCR ivanze-SYBR reagent zitangwa nigikoresho zirashobora kwihuta kandi neza kubona ibisubizo nyabyo-byuzuye bya PCR.

5 × Direct RT ivanze na 2 × Direct qPCR ivanze-SYBR ifite kwihanganira imbaraga za inhibitor, kandi lysate yintangarugero irashobora gukoreshwa nkicyitegererezo cya RT-qPCR itaziguye.Iki gikoresho kirimo RNA idasanzwe-yuzuye ya Foregene reverse transcriptase, hamwe na polymerase ya D-Taq ishyushye, DNTPs, MgCl₂, reaction ya reaction, optimizer ya PCR na stabilisateur.

200 × 20μl Rxns, 1000 × 20μl Rxns

■ Byoroshye kandi byiza: hamwe na tekinoroji ya Direct Direct RT, ingero za RNA zirashobora kuboneka muminota 7 gusa.

Icyitegererezo gikenewe ni gito, nkuko selile 10 zishobora kugeragezwa.

Thr Kwinjiza cyane: irashobora kumenya vuba RNA mu ngirabuzimafatizo zifite 384, 96, 24, 12, 6-amasahani meza.

Er ADN Eraser irashobora gukuraho vuba genome zasohotse, bikagabanya cyane ingaruka kubisubizo byakurikiyeho.

System Optimized RT na qPCR sisitemu ituma intambwe ebyiri RT-PCR ihinduranya inyandiko ikora neza kandi PCR ikarushaho kuba nziza, kandi ikarwanya RT-qPCR inhibitor.

Igipimo cyo gushyira mu bikorwa: ingirabuzimafatizo.

- RNA yarekuwe na sample lysis: ikoreshwa gusa kuri RT-qPCR inyandikorugero yiki gitabo.

- Igikoresho kirashobora gukoreshwa muburyo bukurikira: gusesengura imvugo ya gene, kugenzura ingaruka zo gucecekesha gene ya siRNA, gusuzuma ibiyobyabwenge, nibindi.