Animal Total RNA Isolation Kit
50 Preps, 200 Preps
Chida ichi chimagwiritsa ntchitospin column ndi formulayopangidwa ndi kampani yathu, yomwe imatha kutulutsa RNA yapamwamba komanso yapamwamba kwambiri kuchokera kumagulu osiyanasiyana anyama ndikuchita bwino kwambiri. Imapereka DNA-Cleaning Column yothandiza, yomwe imatha kulekanitsa ndi kutsatsa DNA ya genomic kuchokera ku supernatant ndi lysate ya minofu, yosavuta. ndi kupulumutsa nthawi;Mzere wa RNA wokha ukhoza kumangirira RNA bwino ndipo ukhoza kusinthidwa nthawi imodzi ndi chilinganizo chapadera Zitsanzo zambiri.
Dongosolo lonseli ndi RNase-Free, kotero kuti RNA yochotsedwayo isawonongeke;Buffer RW1, Buffer RW2 buffer makina ochapira, kotero kuti RNA yopezekayo ilibe mapuloteni, DNA, ion, ndi kuipitsidwa kwapawiri.
Zida zamagulu
| Animal Total RNA Isolation Kit | ||
| Zida zamagulu | RE-03011 | RE-03014 |
| 50 T | 200 T | |
| Buffer RL1* | 25ml ku | 100 ml |
| Mtengo wa RL2 | 15ml ku | 60 ml pa |
| Bafa RW1* | 25ml ku | 100 ml |
| Mtengo wa RW2 | 24ml ku | 96ml pa |
| RNase-Free ddH2O | 10 ml pa | 40 ml pa |
| Mzere wa RNA wokha | 50 | 200 |
| DNA-Kuyeretsa Mzere | 50 | 200 |
| Buku Lolangiza | 1 chidutswa | 1 chidutswa |
Zambiri zamalonda
| Mtundu | Mzere wozungulira | Chiyeretso gawo | Foregene column, reagent |
| Flux | 1-24 zitsanzo | Nthawi yokonzekera | ~30 min (24 zitsanzo) |
| Centrifuge | Desk centrifuge | Kupatukana kwa pyrolysis | Kupatukana kwa Centrifugal |
| Chitsanzo | Minofu ya nyama;selo | Zitsanzo kuchuluka | Minofu: 10-20 mg;Selo:(1-5)×106 |
| Elution voliyumu | 50-200 μL | Voliyumu yokweza kwambiri | 850 μl |
Mbali&zabwino
■ Palibe chifukwa chodera nkhawa za kuwonongeka kwa RNA;dongosolo lonse ndi RNase-Free
■ Chotsani bwino DNA pogwiritsa ntchito DNA-Cleaning Column
■ Chotsani DNA popanda kuwonjezera DNase
■ Zosavuta-zochita zonse zimatsirizidwa pa kutentha kwa chipinda
■ Kuthamanga kwachangu kumatha kutha mphindi 30
■ Safe-palibe organic reagent chofunika
■ Kuyera kwakukulu -OD260/280≈1.8-2.1
Kit application
Ndiwoyenera kuchotsedwa ndi kuyeretsedwa kwa RNA yonse kuchokera kumitundu yosiyanasiyana yanyama yatsopano kapena yozizira kapena maselo otukuka.
Zogulitsa katundu
■ Ntchito zotsika: kaphatikizidwe ka cDNA yoyamba, RT-PCR, kupanga maselo, Northern Blot, etc.
■ Zitsanzo: minofu ya nyama, maselo otukuka
■ Mlingo: Tissues 10-20mg, Maselo(2-5)×106
■ Kuchuluka kwa DNA kumangiriza mphamvu yoyeretsa: 80 μg
■ Voliyumu yowonjezera: 50-200 μl
Kuyenda kwa ntchito
Animal Total RNA Isolation Kit ankachitira 20mg
Zitsanzo za mbewa zatsopano, tengani 5% oyeretsedwa okwana RNA 1% agar
Glycogel electrophoresis
1: Nkhumba 2: Impso
3: Chiwindi 4: Mtima
Kusungirako ndi moyo wa alumali
Zidazi zitha kusungidwa kwa miyezi 24 kutentha (15-25 ℃) kapena 2-8 ℃ kwa nthawi yayitali.Buffer RL1 ikhoza kusungidwa pa 4 ℃ kwa mwezi wa 1 mutawonjezera β- mercaptoethanol (posankha).
Nkhani zotchulidwa
1.NGA:18.808:Zheng, Q., Qin, F., Luo, R., et al.mRNA-Loaded Lipid-Monga Nanoparticles kwa Chiwindi Base Editing Kudzera Kukhathamiritsa kwa Central gulu Design.Adv.Ntchito.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.
2.NGATI:18.187:He X, Hong W, Yang J, et al.Apoptosis yodziwikiratu yama cell ochiritsira tsinde yokonzekera imakhala ndi zotsatira zoteteza thupi kutulutsa phosphatidylserine.Signal Transduct Target Ther.2021 Jul 14; 6 (1): 270.doi: 10.1038/s41392-021-00688-z.
3.NGATI: 17.97 
ai Z, Liu H, Liao J, et al.Kusintha kwa N7-Methylguanosine tRNA kumawonjezera kumasulira kwa oncogenic mRNA komanso kumathandizira kupita patsogolo kwa intrahepatic cholangiocarcinoma.Mol cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.
4.NGATI:9.225:Cao X, Shu Y, Chen Y, et al.Kusintha kwa Mettl14-Mediated m6A Kumathandizira Kusinthika kwa Chiwindi Posunga Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.
RNA isoaltion zida za zina zitsanzo magwerozilipo:
Selo, zomera, tizilombo, magazi, etc.
Katswiri waukadaulo wodzipereka kukutsogolerani
Malingana ndi zosowa zanu zenizeni, sankhani njira zomveka bwino zopangira ndi kukonzekera