Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF)，Isoniazid Resistance(INH)
Product name
HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), Isoniazid Resistance (INH) Detection Kit (Melting Curve)
Epidemiology
Mycobacterium tuberculosis, shortly as Tubercle bacillus (TB), is the pathogenic bacterium that causes tuberculosis. Currently, the commonly used first-line anti-tuberculosis drugs include isoniazid, rifampicin and ethambutol, etc. The second-line anti-tuberculosis drugs include fluoroquinolones, amikacin and kanamycin, etc. The new developed drugs are linezolid, bedaquiline and delamani, etc. However, due to the incorrect use of anti-tuberculosis drugs and the characteristics of the cell wall structure of mycobacterium tuberculosis, mycobacterium tuberculosis develops drug resistance to anti-tuberculosis drugs, which brings serious challenges to the prevention and treatment of tuberculosis.
Channel
| Target Name | Reporter | Quencher | ||
| Reaction Buffer A | Reaction Buffer B | Reaction Buffer C | ||
| rpoB 507-514 | rpoB 513-520 | IS6110 | FAM | None |
| rpoB 520-527 | rpoB 527-533 | / | CY5 | None |
| / | / | Internal control | HEX(VIC) | None |
| Reaction Buffer D | Reporter | Quencher |
| InhA promoter region -15C>T, -8T>A, -8T>C | FAM | None |
| KatG 315 codon 315G>A、315G>C | CY5 | None |
| AhpC promoter region -12C>T, -6G>A | ROX | None |
Technical Parameters
| Storage |
≤-18℃ |
| Shelf-life | 12 months |
| Specimen Type | sputum |
| CV | ≤5.0% |
| LoD | The LoD of mycobacterium tuberculosis national reference is 50 bacteria/mL. The LoD of rifampicin-resistant wild type national reference is 2×103 bacteria/mL, and the LoD of mutant type is 2×103 bacteria/mL. The LoD of wild-type isoniazid resistant bacteria is 2×103 bacteria/mL, and The LoD of mutant bacteria is 2×103 bacteria/mL. |
|
Specificity |
The cross test results showed that there was no cross reaction in the detection of human genome, other non tuberculosis mycobacteria and pneumonia pathogens with this kit; There was no cross reaction detected at mutation sites of other drug resistant genes in wild-type Mycobacterium tuberculosis. |
| Applicable Instruments | SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Real-Time PCR System,
Hangzhou Bioer technology QuantGene 9600 Real-Time PCR System, QuantStudio®5 Real-Time PCR System.
|
Total PCR Solution
Professional technical engineer dedicated to guide you
According to your actual needs, choose the most reasonable overall design and planning procedures