50 Preps, 200 Preps

Ka whakamahia e tenei kete tetīwae miro me te tātaii whakawhanakehia e to maatau kamupene, ka taea te tango i te RNA katoa o te parakore me te kounga teitei mai i nga momo momo kararehe me te tino pai. Ka whakaratohia e ia he Tiwae Whakapai DNA pai, ka taea te wehe me te whakauru i te DNA genomic mai i te supernatant me te kiko lysate, ngawari. me te whakaora wa;Ka taea e te Tiwae RNA-anake te here i te RNA ka taea te tukatuka i te wa kotahi me te tauira ahurei He maha nga tauira.

Ko te katoa o te punaha he RNase-Free, kia kore ai te RNA kua tangohia mai e whakaheke;Pūnaha Buffer RW1, Buffer RW2 Buffer washing system, kia kore ai te RNA i whiwhi i te pūmua, te DNA, te katote, me te parahanga pūhui waro.

Nga waahanga kete

Nga korero hua

Nga waahanga me nga painga

■ Kaua e manukanuka ki te whakahekenga o te RNA;he RNase-Free te punaha katoa
■ Kia pai te tango i te Tiwae Horoi-ADN ma te whakamahi i te DNA
■ Tangohia te DNA me te kore e taapiri i te DNase
■ Maamaa-ka oti nga mahi katoa i te pāmahana rūma
■ Ka taea te mahi tere i roto i te 30 meneti
■ Haumaru-kare he reagent waro e hiahiatia ana
■ Te parakore teitei -OD260/280≈1.8-2.1

Te tono kete

He pai mo te tangohanga me te purenga o te RNA katoa mai i nga momo momo kopa kararehe hou, tio ranei, nga pūtau whakatipu.

Tawhā hua

■ Nga tono whakararo: te whenu tuatahi cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Tauira: kopa kararehe, pūtau whakatipu
■ Inenga: Tissues 10-20mg, Pūtau(2-5)×10⁶
■ Te kaha herenga DNA morahi o te pou purenga: 80 μg
■ Rōrahi Elution: 50-200 μl

Te rere mahi

Kararehe Tapeke RNA Isolation Kit i rongoatia 20mg
Ko nga tauira kiore hou, tangohia te 5% RNA katoa kua purea 1% agar

Glycogel electrophoresis
1: Spleen 2: Tākihi
3: ate 4: Ngakau

Te rokiroki me te oranga o te papa

Ka taea te penapena i te kete mo te 24 marama ki te pāmahana rūma (15-25 ℃) 2-8 ℃ ranei mo te wa roa.Ka taea te penapena Buffer RL1 ki te 4 ℃ mo te 1 marama i muri i te taapiri i te β-mercapptoethanol (he kowhiri).

 

Nga tuhinga kua whakahuahia

1.Mena:18.808:Zheng, Q., Qin, F., Luo, R., et al.MRNA-Utaina Lipid-rite Nanoparticles mo te Whakatika turanga ate Na roto i te arotautanga o Central Composite Hoahoa.Adv.Mahi.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.Mena:18.187:He X, Hong W, Yang J, et al.Ko te apoptosis mahorahora o nga pūtau i roto i te whakarite i nga pūtau kakau rongoa ka whai hua immunomodulatory na roto i te tukunga o te phosphatidylserine.Tohu Whakawhiti Whainga Whai.2021 Hūrae 14;6(1):270.doi: 10.1038/s41392-021-00688-z.

3.Mena:17.97 ai Z, Liu H, Liao J, et al.Ko te whakarereketanga o te N7-Methylguanosine tRNA e whakanui ana i te whakamaoritanga mRNA oncogenic me te whakatairanga i te ahunga whakamua o te cholangiocarcinoma intrahepatic.Pūtau Mol.2021 Hūrae 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.Mena:9.225:Cao X, Shu Y, Chen Y, et al.Ko te Mettl14-Mediated m6A Whakarereketanga Ka Whakapaihia te Whakahoutanga Ate ma te pupuri i te Endoplasmic Reticulum Homeostasis.Pūtau Mol Gastroenterol Hepatol.2021;12(2):633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

Ko nga kete wehe RNA mo etahi atu puna tauirakei te wātea:

Pūtau, tipu, viral, toto, etc.

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Tōmua: Pūtau Tika RT QPCR Kit—SYBR GREEN I

Panuku: Ka hangaia e te kaiwhakanao he kiriata whakaata anti-ra

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