Nkwadebe 50, 200 Preps

Ngwa a na-eji yaogho kọlụm na usoronke ụlọ ọrụ anyị mepụtara, nke nwere ike wepụ RNA zuru oke na ịdị ọcha dị elu site na anụ ahụ anụmanụ dị iche iche na-arụ ọrụ dị elu. Ọ na-enye kọlụm DNA na-ehicha nke ọma, nke nwere ike ikewapụ ma gbasaa DNA genomic site na elu na anụ ahụ lysate, dị mfe. na-azọpụta oge;Kọlụm naanị RNA nwere ike jikọta RNA nke ọma ma nwee ike hazie ya n'otu oge site na iji usoro pụrụ iche Ọtụtụ nlele.

Usoro dum bụ RNase-Free, nke mere na RNA amịpụtara adịghị emebi emebi;Buffer RW1, Buffer RW2 sistemu ịsacha ihe, nke mere na RNA enwetara enweghị protein, DNA, ion, na mmetọ ngwakọta organic.

Ngwa ngwa

Ozi ngwaahịa

Atụmatụ & uru

■ Ọ dịghị mkpa ichegbu onwe gị maka mbibi RNA;Sistemụ niile bụ RNase-Free
∎ Wepu DNA nke ọma site na iji kọlụm na-ehicha DNA
■ Wepu DNA na-agbakwunyeghị DNA
■ A na-arụ ọrụ niile dị mfe na ọnụ ụlọ okpomọkụ
■ Enwere ike ịrụcha ọrụ ngwa ngwa n'ime nkeji iri atọ
■ Nchekwa-ọ dịghị ihe reagent organic achọrọ
■ Ịdị ọcha dị elu -OD260 / 280≈1.8-2.1

Ngwa ngwa

Ọ dabara maka mmịpụta na ịdị ọcha nke mkpokọta RNA site na anụ ahụ dị ọhụrụ ma ọ bụ oyi kpọnwụrụ dị iche iche ma ọ bụ sel emebere.

Ngwa ngwaahịa

∎ Ngwa mgbada ala: njikọ cDNA nke mbụ, RT-PCR, cloning molecular, Northern Blot, wdg.
■ Ihe nlele: anụ ahụ anụmanụ, mkpụrụ ndụ omenala
■ Usoro onunu ogwu: Tissues 10-20mg, Sel (2-5) × 10⁶
∎ Oke kachasị ike ijikọ DNA nke kọlụm nchacha: 80 μg
■ Olu olu: 50-200 μl

Usoro ọrụ

Ngwakọta Mkpokọta RNA nke anụmanụ na-agwọ 20mg
Ihe nlele òké ọhụrụ, were 5% RNA 1% agar sachara ọcha

Glycogel electrophoresis
1: Spleen 2: Akụrụ
3: Imeju 4: Obi

Nchekwa na ndụ nchekwa

Enwere ike ịchekwa ngwa ahụ maka ọnwa 24 na okpomọkụ nke ụlọ (15-25 ℃) ma ọ bụ 2-8 ℃ maka ogologo oge.Enwere ike ịchekwa ihe nchekwa RL1 na 4 ℃ maka otu ọnwa ka ịgbakwunye β-mercaptoethanol (nhọrọ).

 

Edemede ndị edepụtara

1.Ọ BỤRỤ: 18.808:Zheng, Q., Qin, F., Luo, R., et al.Lipid-dị ka nanoparticles mRNA-kwadoro maka ndezi imeju site na njikarịcha nke imepụta ihe mejupụtara etiti.Adv.ỌrụMater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.Ọ BỤRỤ: 18.187:He X, Hong W, Yang J, et al.Apoptosis nke mkpụrụ ndụ n'otu n'otu na nkwadebe sel sel ọgwụgwọ na-arụ ọrụ immunomodulatory site na ntọhapụ nke phosphatidylserine.Ebumnuche Ntugharị Mgbaama Ebe ahụ.2021 Jul 14;6 (1):270 .doi: 10.1038/s41392-021-00688-z.

3.Ọ BỤRỤ: 17.97 ai Z, Liu H, Liao J, et al.N7-Methylguanosine tRNA mgbanwe na-akwalite ntụgharị asụsụ mRNA oncogenic ma na-akwalite ọganihu intrahepatic cholangiocarcinoma.Mkpụrụ ndụ Mol2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.Ọ BỤRỤ: 9.225: Cao X, Shu Y, Chen Y, et al.Mettl14-Mediated m6A Mgbanwe na-eme ka mmeghari imeju dị mma site na ijikwa Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021;12 (2): 633-651 .doi: 10.1016/j.jcmgh.2021.04.001.

 

Ngwa iche iche RNA maka isi mmalite sampledị:

Cell, osisi, viral, ọbara, wdg.

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Nke gara aga: Ngwa Cell Direct RT QPCR — SYBR GREEN I

Osote: Onye na-emepụta ihe na-emepụta ihe nkiri na-egbuke egbuke nke anyanwụ

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