Cov khoom siv no siv cov txheej txheem lysis tsis zoo uas tuaj yeem tso tawm RNA sai sai los ntawm cov qauv ntawm tes rau RT-qPCR cov tshuaj tiv thaiv, yog li tshem tawm cov sijhawm siv sijhawm thiab siv zog RNA purification txheej txheem.Tus qauv RNA tuaj yeem tau txais hauv 7 feeb xwb.Lub 5 × Direct RT Mix thiab 2 × Direct qPCR Mix-SYBR reagents muab los ntawm cov khoom siv tuaj yeem tau txais cov txiaj ntsig PCR sai thiab ua tau zoo.

5 × Direct RT Mix thiab 2 × Direct qPCR Mix-SYBR muaj zog inhibitor kam rau ua, thiab lysate ntawm cov qauv siv tau los ua tus qauv rau RT-qPCR ncaj qha.Cov khoom siv no muaj qhov tshwj xeeb RNA high-affinity Foregene reverse transcriptase, thiab Kub D-Taq DNA polymerase, dNTPs, MgCl₂, cov tshuaj tiv thaiv tsis zoo, PCR optimizer thiab stabilizer.

200 × 20μl Rxns, 1000 × 20μl Rxns

■ Yooj yim thiab siv tau: nrog Cell Direct RT thev naus laus zis, cov qauv RNA tuaj yeem tau txais hauv 7 feeb xwb.

■ Cov qauv xav tau me me, tsawg li 10 lub hlwb tuaj yeem kuaj tau.

■ Kev xa tawm siab: nws tuaj yeem ntes RNA sai sai hauv cov kab mob hauv 384, 96, 24, 12, 6-zoo daim hlau.

■ DNA Eraser tuaj yeem tshem tawm cov genomes sai sai, txo qhov cuam tshuam rau cov txiaj ntsig kev sim tom ntej.

■ Optimized RT thiab qPCR system ua rau ob-kauj ruam RT-PCR thim rov qab tau zoo dua thiab PCR ntau dua, thiab tiv taus ntau dua rau RT-qPCR cov tshuaj tiv thaiv.

Scope ntawm daim ntawv thov: kab lis kev cai.

- RNA tso tawm los ntawm cov qauv lysis: tsuas yog siv tau rau RT-qPCR qauv ntawm cov khoom siv no.

- Cov khoom siv tuaj yeem siv rau cov hom phiaj hauv qab no: kev tshuaj ntsuam gene qhia, kev txheeb xyuas ntawm siRNA-mediated gene silencing effect, tshuaj ntsuam xyuas tshuaj, thiab lwm yam.