50 Preps, 200 Preps

Cov khoom siv no siv lubspin kem thiab formulatsim los ntawm peb lub tuam txhab, uas muaj peev xwm rho tawm high-purity thiab high-zoo tag nrho RNA los ntawm ntau yam tsiaj cov ntaub so ntswg nrog high efficiency.It muab ib tug npaum DNA-Cleaning Kem, uas tau yooj yim cais thiab adsorb genomic DNA los ntawm supernatant thiab cov ntaub so ntswg lysate, yooj yim. thiab txuag lub sijhawm;RNA nkaus xwb Kem tuaj yeem khi RNA zoo thiab tuaj yeem ua tiav ib txhij nrog cov qauv tshwj xeeb Ntau cov qauv.

Tag nrho cov kab ke yog RNase-Free, kom cov RNA rho tawm tsis degraded;Buffer RW1, Buffer RW2 tsis siv lub tshuab ntxhua khaub ncaws, kom cov RNA tau txais tsis muaj protein ntau, DNA, ion, thiab organic compound pollution.

Cov khoom siv

Cov ntaub ntawv khoom

Nta & qhov zoo

■ Tsis tas yuav txhawj txog RNA degradation;tag nrho cov kab ke yog RNase-Free
■ Tshem tawm DNA-siv DNA-Tshuaj Kem
■ Tshem tawm DNA yam tsis tau ntxiv DNase
■ Kev ua haujlwm yooj yim-txhua yam ua tiav ntawm chav tsev kub
■ Ceev -kev ua haujlwm tuaj yeem ua tiav hauv 30 feeb
■ Kev nyab xeeb-tsis muaj cov organic reagent yuav tsum tau
■ High purity -OD260/280≈1.8-2.1

Daim ntawv thov Kit

Nws yog qhov tsim nyog rau kev rho tawm thiab ua kom huv ntawm tag nrho RNA los ntawm ntau yam tshiab lossis khov tsiaj cov ntaub so ntswg lossis kab lis kev cai.

Khoom parameters

■ Cov ntawv thov hauv qab: thawj-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, thiab lwm yam.
■ Cov qauv: cov ntaub so ntswg tsiaj, cov kab lis kev cai
■ Siv: Cov ntaub so ntswg 10-20mg, Cells (2-5) × 10⁶
■ Qhov siab tshaj plaws DNA khi muaj peev xwm ntawm purification kem: 80 μg
■ Elution ntim: 50-200 μl

Kev khiav haujlwm

Animal Total RNA Isolation Kit kho 20mg
Cov qauv nas tshiab, noj 5% purified tag nrho RNA 1% agar

Glycogel electrophoresis
1: Spleen 2: Lub raum
3: Lub siab 4: Lub siab

Cia thiab txee lub neej

Cov khoom siv tuaj yeem khaws cia rau 24 lub hlis ntawm chav tsev kub (15-25 ℃) lossis 2-8 ℃ rau lub sijhawm ntev.Buffer RL1 tuaj yeem khaws cia ntawm 4 ℃ rau 1 lub hlis tom qab ntxiv β-mercaptoethanol (optional).

 

Cov lus hais

1.PIB: 18.808.Zheng, Q., Qin, F., Luo, R., et al.mRNA-Loaded Lipid-zoo li nanoparticles rau lub siab Base Editing ntawm Optimization ntawm Central Composite Design.Adv.Ua haujlwm.Mater.2021, 31, 2011068.doi:10.1002/adfm.202011068.

2.PIB: 18.187.He X, Hong W, Yang J, et al.Spontaneous apoptosis ntawm cov hlwb nyob rau hauv kev kho mob qia cell npaj exert immunomodulatory teebmeem los ntawm kev tso tawm ntawm phosphatidylserine.Teeb liab Transduct Target Ther.2021 Lub Xya Hli 14; 6(1): 270.doi: 10.1038/s41392-021-00688-z.

3.Ib: 17.97 Liu Z, Liu H, Liao J, et al.N7-Methylguanosine tRNA kev hloov kho txhim kho oncogenic mRNA kev txhais lus thiab txhawb kev loj hlob ntawm intrahepatic cholangiocarcinoma.Mol Cell.2021 Jul 29:S1097-2765(21)00555-4.doi: 10.1016/j.molcel.2021.07.003.

4.Ib: 9.225: Cao X, Shu Y, Chen Y, et al.Mettl14-Mediated m6A Kev Hloov Kho Ua kom lub siab rov tsim dua tshiab los ntawm kev tuav cov Endoplasmic Reticulum Homeostasis.Cell Mol Gastroenterol Hepatol.2021; 12(2): 633-651.doi: 10.1016/j.jcmgh.2021.04.001.

 

RNA isoaltion cov khoom siv rau lwm yam qauvmuaj:

Cell, cog, kab mob, ntshav, thiab lwm yam.

---

Yav dhau los: Cell Direct RT QPCR Kit—SYBR GREEN I

Tom ntej: Cov chaw tsim khoom tsim cov yeeb yaj kiab tiv thaiv tshav ntuj

---