Colloidal gold is a commonly used labeling technology. It is a new type of immunolabeling technology that uses colloidal gold as a tracer marker for antigens and antibodies. It has unique advantages. In recent years, it has been widely used in various biological studies. Almost all immunoblotting techniques used clinically use their label. At the same time, it can be used in flow cytometry, electron microscopy, immunology, molecular biology, and even biochips.
Colloidal gold labeling is essentially a coating process in which proteins such as proteins are adsorbed onto the surface of colloidal gold particles. The adsorption mechanism may be the negative charge on the surface of the colloidal gold particles, which forms a strong bond with the positively charged groups of the protein due to electrostatic adsorption. Colloidal gold particles of various particle sizes, that is, different colors, can be easily prepared from chloroauric acid by reduction method. This spherical particle has a strong adsorption function for proteins and can be non-covalently combined with staplococcal protein A, immunoglobulins, toxins, glycoproteins, enzymes, antibiotics, hormones, bovine serum albumin polypeptide conjugates, Therefore, it has become a very useful tool in basic research and clinical experiments.
Colloidal gold technology has the advantages of convenience, quickness, specific sensitivity, strong stability, no special equipment and reagents, and intuitive judgment of results. And broad application prospects.